|
KMID : 1007520070160050854
|
|
Food Science and Biotechnology
2007 Volume.16 No. 5 p.854 ~ p.857
|
|
|
Phenolic Antioxidants Isolated from Mulberry Leaves
|
|
Kim Young-Chan
Kim Mi-Yeon
Takaya Yoshiaki
Niwa Masatake
Chung Shin-Kyo
|
|
|
Abstract
|
|
|
|
In this study, the crude methanol extract of mulberry leaves was fractionated with chloroform, ethyl acetate, n-butanol, and water, successively. The antioxidant activities of the fractions were examined with the 2-deoxyribose oxidation and linoleic acid peroxidation methods. The ethyl acetate fraction showed the strongest antioxidant activity. From it we isolated chlorogenic acid, caffeic acid, quercetin 3-O-β-D-glucopyranoside, and kaempferol 3-O-β-D-glucopyranoside with preparatory octadecyl silane-high performance liquid chromatography (ODS-HPLC), and identified the compounds by nuclear magnetic resonance (NMR) and fast atom bombardment mass (FAB-MS) analyses. Overall, quercetin 3-O-β-D-glucopyranoside showed the strongest antioxidant activity by both the 2-deoxyribose oxidation and rat liver microsome peroxidation methods.
|
|
|
KEYWORD
|
|
|
quercetin 3-O-β-D-glucopyranoside, chlorogenic acid, mulberry Leave, antioxidant activity
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|
|